![]() wrong exon/intron limits, splitted genes, or merged genes. If you inspect some predicted genes, you will probably find some mistakes made by Maker, e.g. You can do it by using Blast, InterProScan, or Blast2GO for example.Īn automatic annotation of an eukaryotic genome is rarely perfect. ![]() What’s next?Īfter generating your annotation, you will probably want to automatically assign functional annotation to each predicted gene model. ConclusionĬongratulations, you finished this tutorial! You learned how to annotate an eukaryotic genome using Maker, how to evaluate the quality of the annotation, and how to visualize it using the JBrowse genome browser. Param-files “GFF/GFF3/BED Track Data”: select the output of Map annotation ids Tool: toolshed.g2.bx.psu.edu/repos/iuc/maker_map_ids/maker_map_ids/2.31.11Įnable the track on the left side of JBrowse, then navigate along the genome and look at the genes that were predicted by Maker.
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